Hamilton Ion Exclusion HPLC Columns
Ion exclusion chromatography is a technique applied to separate ionic from nonionic compounds and mixtures of organic acids and alcohols. The electric charge sign of the dissociated functional groups of the ion exchange resin is the same as the electric charge sign of the analyzed ionic
compound, excluding them from the pores of the support resin and eluting first while the nonionic species elute later.
Mixtures of weak organic acids, like those in fruits and milk products, are frequently not very well separated by pure ion exchange methods, nor in the reversed-phase mode. Hamilton offers a polymeric packing material (PRP-X300) for ion exclusion separations which effectively separates these mixtures.
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